Scribe Therapeutics is a molecular engineering company focused on creating best-in-class in vivo therapies that permanently treat the underlying cause of disease. Founded by CRISPR inventors and leading molecular engineers Benjamin Oakes, Brett Staahl, David Savage, and Jennifer Doudna, Scribe is overcoming the limitations of current genome editing technologies by developing custom engineered enzymes and delivery modalities as part of a proprietary, evergreen CRISPR by Design™ platform for CRISPR-based genetic medicine.
We are seeking a Scientist with experience in RNA biochemistry and an adaptable skillset of computational, biophysical, and biochemical techniques to probe the structure/function of single-guide RNAs (sgRNAs). The candidate will join the Process Science analytical team to study RNA folding computationally and experimentally for its CRISPR by Design™ platform. The ideal candidate should have a good understanding and knowledge of RNA biochemistry and methods for probing RNA structural ensembles. We are seeking experienced, enthusiastic, creative, and highly motivated individuals who are comfortable working in a fast-paced entrepreneurial CMC environment.
Key Responsibilities:
- Structure probing of development candidate sgRNAs using enzymatic or chemical structure mapping techniques such as DMS MaP-seq and/or high-throughput SHAPE
- NGS library design, preparation, QC, and pipeline construction for NGS
- In-silico RNA structural modeling and alternative RNA structure ensemble deconvolution
- Construction of cloud-based computational environments for RNA structure analysis
- sgRNA sequencing for identity confirmation by NGS
- Biophysical studies of sgRNA binding affinity for CasX using Biolayer Interferometry (BLI)
- Biophysical studies of DNA target-binding affinity of CRISPR-CasX effector complexes using BLI
- Functional characterization (in vitro DNA cleavage) of differential CRISPR-Cas RNP complexes by electrophoretic mobility shift assay (EMSA) or anion exchange (AEX) chromatography
- Work collaboratively in a multi-disciplinary environment with Process Science and Research teams
- Maintain detailed records of experimental methods and data in electronic laboratory notebook (Benching)
Required Skills and Background:
- PhD with 0-2 years of experience, or MS with 6+ years of experience in an academic/ industry setting. Degree in Chemistry, Biochemistry, Chemical Engineering, Bioengineering, Pharmaceutical Sciences, or related discipline
- Excellent oral and written communication skills
- Effective organizational and time management skills
- Strong focus and attention to detail
- Ability to work both independently and collaboratively in a fast-paced, interdisciplinary research team
Preferred Skills and Background:
- Familiarity with CRISPR technologies and therapeutic approaches
- In silico RNA folding / prediction algorithms
- RNA biochemistry
- Experience in NGS based assay development and implementation
- Hands-on experience with Illumina, PacBio, and/or Nanopore sequencing platforms
- Bioinformatic pipelines; Python, MATLAB
Salary will be commensurate with experience. We will provide an intellectually stimulating, collegial and fast-paced environment. If you are ready to engineer the future of therapeutics, then we are excited to hear from you! Visit us at www.scribetx.com.
We are committed to creating a diverse environment and are proud to be an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, gender, gender identity or expression, sexual orientation, national origin, disability, age, or veteran status.
At the time of posting, the base pay wage range for this role is $100,000-$130,000 per year. The offered pay range will depend on internal equity and the candidate’s relevant skills, experience, qualifications, training, and market data. Additional incentives are provided as part of the complete package in addition to comprehensive medical and other benefits.
